In PART 1, you replicated DNA but did not change any settings.  In PART 2, your GOAL is to investigate the effects of changing variables in the simulation on your DNA replication.  To complete this goal, you will need to complete a second set of CHALLENGES.

[NOTE:  If at any time in Challenges 5 through 7, you “run out of DNA” to replicate, just reset your simulation and continue with your challenge.]

1. Start by specifying the following settings in the simulator:

DNA-strand-length:  30                               Speed:  see below

Enzyme labels: checked                              Substitutions:  unchecked

Nucleo labels: unchecked                            Free-nucleosides:  150                    Time-limit:  No Limit

2. When you have chosen the correct settings, press SETUP. Press GO/STOP. 

3. Start DNA replication by unwinding and unzipping your DNA and attaching polymerase ONLY on the TOP strand.

4. First change the simulation speed by moving the slider to the left, to FASTER.

Do NOT move the nucleosides using mouse. Observe the random motion of nucleosides and DNA replication. 

6. After you replicate the top strand, decrease the simulation speed by moving the slider to the left, to SLOWER.

Attach the polymerase to the bottom strand.

Now, observe the replication bottom strand of DNA.  Press GO/STOP to end the simulation.